Protease Liquid Enzyme

PROTEAZ 150 protease is a high-strength liquid proteolytic enzyme solution for use in liquid laundry detergents. This product is a very efficient detergent protease that provides good performance over a broad range of application conditions, including liquid detergents of various compositions and in wash temperatures in the range 25 – 60°C (68 – 144°F).

PROTEAZ 150 protease is a genetically modified bacterial endoprotease, which is an enzyme that hydrolyzes insoluble protein stains into soluble peptides in the interior of a protein chain. These hydrolysis products are small polypeptides that are then more easily removed during the washing process.

PROTEAZ 150 protease is particularly useful in removing common household stains such as blood, grass, milk, and food residues from soiled surfaces. The product is highly concentrated, has excellent color and stability in detergent formulations, and is conveniently handled and dosed.

PROPERTIES

PROTEAZ 150 protease is active over a broad range of pH values from 7 – 12 with optimum activity in the pH range 9 – 11, as depicted in Figure 1. Product performance testing has also resulted in good performance at lower pH levels.

PROTEAZ 150 protease exhibits maximum activity at about 50° C (122° F). Application studies have shown that PROTEAZ 150 protease has excellent performance characteristics within a broad temperature range (25 – 60°C) [68 – 144°F].

UNITS OF ACTIVITY

The proteolytic activity of PROTEAZ 150 enzyme is expressed in Properase Units (PU). The activity determination method is a modification of the Alkaline Delft Unit (ADU) method and is based on the proteolytic hydrolysis of casein at pH 10.

USAGE IN DETERGENT FORMULATIONS

Stability in Detergent Formulations

The stability of PROTEAZ 150 protease is determined by the composition of each individual liquid detergent. PROTEAZ 150 protease offers storage stability characteristics identical to or better than those of conventional proteases.

Several factors influence the stability of this enzyme product.

The following general rules apply:

• High ratio of nonionics to ionics is preferable
• Water content below 40% is advisable
• Certain ions, such as Ca++, positively influence enzyme stability

Depending on the particular formulation of the liquid detergent, over 85% residual activity is available after 6 weeks’ storage at 30° C (94° F).

Concentration

The determination of the exact amount of PROTEAZ 150 protease required for laundry applications should be based upon wash conditions, detergent formulae, amount of detergent used per wash, and the level of cleaning performance desired. Small-scale tests should be performed to determine the appropriate enzyme dosage before production begins. Actual dosing is easily accomplished using volumetric dosing equipment.

Compatibility with Detergent Ingredients

PROTEAZ 150 protease shows excellent performance characteristics in a wide variety of detergent formulations and is compatible with other enzymes, such as lipases, amylases and cellulases.

SAFETY & ENZYME HANDLING

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes.

For detailed handling information, please refer to the appropriate Material Safety Data Sheet

PACKAGING & STORAGE STABILITY

PROTEAZ 150 enzyme is available in 25 liter pails and 225 liter drums..

Under normal handling and storage conditions, PROTEAZ 150 enzyme remains stable for at least four months. To retain maximum enzyme activity, this product should be stored at moderate temperatures in the original container with the lid closed. Shelf life is increased when the product is stored at lower temperatures.